Protein
phosphorylation plays an unusually prominent role in cell
signaling, development and growth. The RayBio® Human RTK
Phosphorylation antibody array kits are a very rapid, convenient
and sensitive assay to simultaneously detect multiple protein
phosphorylation and can be used to monitor activation or
function of important biological pathways. RayBiotech is
committed to develop a series of phosphorylation antibody arrays.
Our first product in this series is RayBio® Human RTK
Phosphorylation antibody array I, which is specifically designed
for simultaneously identifying the relative levels of
phosphorylation of seventy-one different Human Receptor Tyrosine
Kinases (RTKs) in cell lysate. By monitor changes in protein
tyrosine phosphorylation in your experimental model system, you
can verify pathway activation in your cell lines without
spending excess time and effort in performing an analysis of
immunoprecipitation and/or Western Blot.
By using
RayBio®
Human
RTK Phosphorylation
antibody array I, treated or untreated cell lysate is added into antibody
array chip (either membranes or glass slides). The antibody
array chips are washed and biotinylated anti-phosphotyrosine
antibody is used to detect phosphorylated tyrosines on activated
receptors. After incubation with HRP-streptavidin or Alexa Fluor
555-streptavidin, the signals are visualized by
chemiluminescence or fluorescence imaging system. |
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71
Receptor Tyrosine Kinases List: |
|
ABL1 |
ACK1 |
ALK |
Axl |
Blk |
BMX |
|
Btk |
Csk |
Dtk |
EGFR |
EphA1 |
EphA2 |
|
EphA3 |
EphA5 |
EphA6 |
EphA7 |
EphA8 |
EphB1 |
|
EphB2 |
EphB3 |
EphB4 |
EphB5 |
EphB6 |
ErbB2 |
|
ErbB3 |
ErbB4 |
FAK |
FER |
FGFR1 |
FGFR2 |
|
FGFR2 (α isoform) |
Fgr |
FRK |
Fyn |
Hck |
HGFR |
|
IGF-I R |
Insulin R |
Itk |
JAK1 |
JAK2 |
JAK3 |
|
LCK |
LTK |
Lyn |
MATK |
M-CSFR |
MUSK |
|
NGFR |
PDGFR-α |
PDGFR-β |
PYK2 |
RET |
ROR1 |
|
ROR2 |
ROS |
RYK |
SCFR |
SRMS |
SYK |
|
Tec |
Tie-1 |
Tie-2 |
TNK1 |
TRKB |
TXK |
|
Tyk2 |
TYRO10 |
VEGFR2 |
VEGFR3 |
ZAP70 |
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RayBio®
Human RTK Phosphorylation Antibody Array (membrane-based):
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RayBio®
Phosphorylation Antibody Array (glass-chip based):
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Example of
Potential Applications: |
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Monitoring cell
signal transduction pathway, relative level of tyrosine
phosphorylation of RTKs and protein kinase activity |
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Identifying crucial factors involved in disease process |
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Discover biomarkers for diagnosis and prognosis, involved in
drug development |
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Screening the effects of various treatments, activators or
inhibitors in cell signaling, development and growth
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1658 9045
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