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Key points for ELISA
Dilution of condensed reagents - standard solution, Labeled antibody solution
Washing of microplate - Dilution of washing buffer, High bank absorbance, Low or too much colouration, Peeling off of capture antibody
Shaking of microplate - Low colouration, Big variation, Poor sensitivity, 600 ~ 1,200rpm, 10sec.x3 times
Assay circumstance - Room temp(20~25 Celsius), Humidity(>30%), Airflow(0,4m/sec.>), Level, Avoidance of direct sunlight
Precaution to the safety - Gloves, Glasses, Mask, Laboratory Coats, 1M Sulphuric Acid, Assay Sample handling, Disposal of materials after assays, Storage of kits and term of validity
Instrumentation - Beaker-Graduated cylinder, Pipette - Tips, Vortex-type mixer, Thermometer-Hygrometer, Timer, Microplate reader(450nm, 620nm), Test tubes, Test rucks, others
Temperature adaptation - Temperature Adaptation Time, For partial use, TMB
Handling of pipette - Touch&Go, Pre-wetting, Co-washing
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We would like to inform you that the highly sensitive Rat Thyroid Stimulating Hormone ELISA Kits from Shibayagi is available again and is quickly taking over the biological sphere.
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These assays were unfortunately discontinued in 2012, but now Shibayagi released the new and improved version of those assays.
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It is the most sensitive Rat TSH ELISA on the market. It uses 2 2 monoclonal antibodies directed to the α- and β-subunits of the TSH molecule which is a heterodimer.
Reference values:
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Male CD (SD) rats, 9 weeks of age, fasting, TSH values: 3,26~4,65 ng/ml , n=5
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Male CD (SD) rats, 7 months of age, non-fasting, TSH values: 2,50~4,04 ng/ml, n=5
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Male CD (SD) rats, 9 weeks of age, non-fasting, thyroidectomized, TSH values: 8,09~11,9 ng/ml , n=5
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+32
1641 4407
+32
1650 9045 
