Nitric oxide
(NO) plays an important role in neurotransmission, vascular
regulation, immune responses and apoptosis. Since
most of the NO is oxidized to nitrite (NO2-)
and nitrate (NO3-), the concentrations
of these anions have been used as a quantitative measure of
NO production.
BioVision’s
Nitric Oxide Fluorometric (Cat# K252-200)
and
Colorimetric
(K262-200)
Assay Kits provide an accurate and convenient measurement of
total nitrate/nitrite concentration in a simple two-step
process. The first step is the conversion of nitrate to
nitrite utilizing nitrate reductase. For fluorometric assay,
the second step involves addition of DAN followed by NaOH,
which converts nitrite into a fluorescent compound.
Measurement of the fluorescence of this compound accurately
determines the total nitric oxide production. For
colorimetric assay, the second step involves addition of the
Griess Reagents which convent nitrite into a deep purple azo
compound. Measurement of the absorbance of the azo
chromophore accurately determines the total nitric oxide
production. Both kits have been validated in culture media,
plasma, and tissue homogenates. Some sample purification
from these sources may be necessary.
+32
1658 9045
+32
1650 9045
