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MAGNETIC SEPARATORS
Spherotech has several different designs of
magnetic separators. These are used for separating both Paramagnetic and
Ferromagnetic particles from a suspension. These designs accommodate
different size tubes and other receptacles specific to various
applications.
(A) The SPHEROTM FlexiMag Separator (Cat. No.:
FMS-1000) accommodates tubes ranging from 1.5 mL microfuge tube to 200
mL tissue culture bottle. It uses interchangeable tube holders to secure
different size tubes and bottles. The separator comes with a set of
three tube holders, Small, Medium and Large. Additional holders can be
purchased separately, if needed.
a) The Small holder holds up to four 1.5 mL
microfuge or four 10 or 12x75 mm test tubes (8 total).
b) The Medium holder can hold two 15 mL
centrifuge or two 16x100 mm tubes (4 total)
c) The Large holder can hold two 50 mL
centrifuge tubes (4 total).
d) Two 200 mL tissue culture bottles directly
without any tube holders.
(B) The SPHEROTM FlexiMag Separator Jr. (Cat.
No.: FMJ-1000) holds up to eight 1.5 mL microfuge tubes, 5 mL cryovials,
10x75 mm or 12x75mm tubes.
(C) The SPHEROTM MicroMag Separator (Cat No.
MMS-2100 and MMS-2000) are designed to fit any 96-well plate with round
bottom, flat bottom or V bottom.
(D) The SPHEROTM HandiMag Separator is a
1"x2"x0.375" Neodymium-Iron-Boron high strength magnet with nickel
coating . The HandiMag can be used to separate the magnetic particles in
various containers such as microfuge tubes, test tubes or centrifuge
tubes by holding the magnet against the wall of containers by hand or
with a rubber band.
(E) The SPHEROTM MiniTube Mag Separator (Cat.
No.: MTMS-16) holds up to sixteen 1.5 mL tubes.
(F) The SPHEROTM UltraMag Separator (Cat. No.:
UMS-3000) and SPHEROTM UltraMag DW Separator
(Cat. No.: UMDS-1000) are designed to
facilitate the washing of magnetic particles in the Magnetic Particles
Enzyme Immunoassay (MPEIA) using 96-well
plates. The magnetic pegs of UltraMag Separator fit between the wells
underneath the 96-well plate. The microplate is read in an appropriate
microtiter plate reader by loading the microplate with the attached
UltraMag Separator into the reader. The magnets ensure that the magnetic
particles stay out of light beam passing through the bottom of the well
and corresponding holes in the UltraMag Separator.
A combination of magnetic particles as solid
phase and conventional enzyme immuno assay (EIA) microplate technology
offers significant advantages over the conventional EIA. Some of the
advantages are listed below:
• Magnetic particles offer larger surface area
and significantly faster reaction kinetics; thus, reducing the total
time to complete an assay.
• Microparticles are washed more efficiently
leaving less residual reactants, thus, lowering background signal and
potentially improving sensitivity.
• Coating of magnetic particle is easier, gives
a more uniform solid phase and helps to minimize lot-to-lot variation.
See the MAGNETIC SEPARATOR section of the
TECHNICAL INFORMATION Page of the Spherotech website to see photos of
several magnetic separators.
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+32
1658 9045
+32
1650 9045
