The regulation
of HDL (high-density-lipoprotein)-cholesterol and LDL (low-density-lipoprotein)-cholesterol
plays a central role in various disease development.
It is well known that low levels of HDL and high level of
LDL are associated with an increased risk of cardiovascular
events. The HDL and LDL/VLDL Cholesterol Quantification Kit
provides a simple method for convenient separation of HDL
from LDL and VLDL (very low-density lipoprotein) in serum
sample, then HDL and LDL/VLDL cholesterol levels can easily
be quantified separately, using cholesterol oxidase mediated
reactions. Cholesterol esterase hydrolizes cholesteryl ester
into free cholesterol, which can then be oxidized by
cholesterol oxidase to yield H2O2. The
H2O2 interacts with a sensitive
cholesterol probe to produce resorufin, which can be
detected with a micro-plate reader at l = 570 nm or
fluorescence micro-plate reader at Ex/Em = 538/587 nm. The
assay can also detect cholesterol ester and free cholesterol
separately in these two fractions by adding or not adding
cholesterol esterase into the reactions.
Measurement
of total cholesterol, HDL, LDL/VLDL from serum
samples. Total Cholesterol (dark bar), HDL (shadow
Bar), and LDL/VLDL (open Bar) cholesterol was
measured follow the kit protocol.
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