Glutathione S-transferase
(GST) is a family of enzymes that play an important role in
detoxification of xenobiotics. GST catalyzes the
formation of the thiol group of glutathione to electrophilic
xenobiotics. It utilizes glutathione to scavenge potentially
toxic compounds including those produced as a result of
oxidative stress and is part of the defense mechanism
against the mutagenic, carcinogenic and toxic effects of
such compounds.
Standard
Calibration Curve of GST Measured by
spectrophotometry. Various amounts of standard
GST was incubated with GSH and CDNB according to the
kit instructions. Absorbances were measured at 340
nm.
Inhibition of GST Activities by GST Inhibitors.
Various amounts of GST Inhibitor-1 (Cat.
1555-1000) or GST Inhibitor-2 (Cat. 1556-1000)
were added to GST assay reactions. GST activity
was measured by GST Fluorometric Assay Kit (Cat.
K260-100) according to the kit instructions.
+32
1658 9045
+32
1650 9045
