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Europe
GENTAUR
 +32
1658 9045
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1650 9045
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Glutathione is
the major intracellular low-molecular-weight thiol.
It plays a critical role in cellular defense against
oxidative stress in tissues and cells. BioVision offers
three sensitive, easy-to-use glutathione detection kits. The
Glutathione Fluorometric Detection Kit (K251-100)
utilizes monochlorobimane (MCB), a dye that has a
high affinity for glutathione, to measure the change of
glutathione level during pathological processes (e.g.,
apoptosis). The Glutathione Colorimetric Detection
Kit (K261-100) utilizes the glutathione recycling
system by DTNB and glutathione reductase to quantify total
glutathione and the reduced form of glutathione. The
recycling system dramatically improves the detection
sensitivity. The novel Oxidized, Reduced, and Total
Glutathione Detection Kit (K264-100) utilizes
special formulations of buffers and reagents to quantify the
oxidized, reduced, and total glutathione separately. This is
the only commercially available kit that can detect oxidized
and reduced glutathione individually.Glutathione
Reductase Assay Kit (K761-200) is also available.
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Product |
Cat. # |
Size |
Data Sheet
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Glutathione Fluorometric Detection Kit |
K251-100 |
100 assays |
Product Data Sheet |
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The kit provides a fluorescence method
to detect the change in glutathione level in apoptotic cells
or other pathological processes using a fluorometer or a 96-well
fluorometric plate reader (Ex/Em = 380/460 nm).
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Product |
Cat. # |
Size |
Data Sheet
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Glutathione Colorimetric Detection Kit |
K261-100 |
100 assays |
Product Data Sheet |
The kit provides a colorimetric method
to analyze the total glutathione and/or the reduced form of
glutathione by microtiter plate reader ( =
405 or 415 nm). The recycling system utilized in the assay
dramatically improves the detection sensitivity.
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Product |
Cat. # |
Size |
Data Sheet
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Glutathione Assay Kit (GSH, GSSG and Total) |
K264-100 |
100 assays |
Product Data Sheet |
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The novel glutathione assay kit detects
the GSH, GSSG, and total glutathione individually using a
fluorescence method (Ex/Em = 340/450 nm) in 96 well
microtiter plates. The kit also provides unique procedure
and buffer formula to eliminate protein thiol interference
and thus stabilize GSH and GSSG in solutions. The assay is
easy to perform and detects 2-400 ng/ml of GSH, GSSG or
total glutathione.
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Name |
Cat. # |
Size |
Data Sheet
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Glutathione Reductase Assay Kit |
K761-200 |
200 assays |
Product Data
Sheet |
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This Assay Kit is a highly sensitive,
simple, direct and HTS-ready colorimetric assay for measuring GR
activity in biological samples. In the assay, GR reduces GSSG to
GSH, which reacts with 5, 5′-Dithiobis (2-nitrobenzoic acid) (DTNB)
to generate TNB2- (yellow color, -max = 405 nm). The assay can
detect 0.1 – 40 mU/ml GR in various samples. |
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Name |
Cat. # |
Size |
Data Sheet
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Glutathione Peroxidase Assay Kit |
K762-100 |
100 assays |
Product Data
Sheet |
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Glutathione Peroxidase Assay Kit measures
glutathione peroxidase (GPx) activity through a coupled reaction
with glutathione reductase (GR). In the assay, GPx reduce Cumene
Hydroperoxide, and oxidize GSH to GSSG. The generated GSSG is
reduced to GSH with consumption of NADPH by GR. The decrease of
NADPH is proportionally to GPx activity in the reactions. The
decrease of NADPH can be easily measured by absorbance at 340
nm. The assay can be used to measure all of the glutathione
dependent peroxidases in plasma, erythrocyte lysates, tissue
homogenates, and cell lysates with detection sensitivity ~ 0.5
mU/ml of GPx in samples. |
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Name |
Cat. # |
Size |
Data Sheet
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Thioredoxin Reductase Assay Kit |
K763-100 |
100 assays |
Product Data
Sheet |
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BioVision’s Thioredoxin Reductase Assay Kit
provides a convenient colorimetric assay for detecting TrxR
activity in various samples. In the assay TrxR catalyzes the
reduction of 5, 5’-dithiobis (2-nitrobenzoic) acid (DTNB) with
NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generate a
strong yellow color (λmax = 405 nm). |
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K251-100 GSH Level Decreases
Following Induction of Apoptosis.
Jurkat cells were treated with
camptothecin for 8 hours. GSH level
was measured using the ApoGSHTM
Fluorometric kit according to kit
instructions.
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K264-100
Glutathione assays were prepared
using standard GSH and GSSG
following the kit instructions.
Diamond (blue) represents GSH
standard curve. Square (pink)
represents standard curve produced
by adding GSSG + GSH Quencher +
Reducing Agent Mix. Triangle (gray)
represents GSS92G standard without
adding Reducing Agent mix.
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K261-100 Glutathione Standard
Calibration Curve.
10 minutes incubation at room
temperature.
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Pricelist
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