BioVision offers
several novel kits for fast and sensitive detection of
DNA ladder in apoptotic cells. Unlike other similar kits
in the market which require 1-2 days to prepare nucleic
acids, the new procedure requires less than 90 minutes to
prepare chromosomal DNA, requiring neither extraction
nor a column. The newly released Apoptotic DNA ladder
extraction kit can detect apoptotic DNA ladder from tissue
and cells that contain even 5% apoptotic cell population.
DNA ladders can easily be visualized by agarose gel
electrophoresis.
The TUNEL-based DNA fragmentation assay kits provide
complete components including positive and negative control
cells or tissue slides for convenient detection of DNA
fragmentation in cultured cells and tissue sections. Results
can be analyzed by flow cytometry or fluorescence microscopy.
Quick Detection
of Apoptotic DNA Ladder in Jurkat Cells.
Apoptosis was induced in Jurkat cells
with camptothecin (2 mM) for 0 hr (Lane
1), 6 hrs (Lane 2) and 12 hrs (Lane 3).
Chromosomal DNA was prepared using the
Quick Apoptotic DNA Ladder Detection Kit
according to the kit instructions. 20 ml
of each sample was electrophoresed on a
1.2% agarose/EtBr gel.
Detection of
Apoptotic Cells Using Apo-BrdUTM Kit by
Flow Cytometer. Apoptosis was
induced in Jurkat cells with
camptothecin (2 mM) for 8 hours. Control
cells (uninduced) and induced cells were
both collected, labeled with ApoBrdU
according to the kit instructions.
Results were analyzed by flow cytometry.
Provides a simple procedure
to prepare chromosomal DNA less than 90 minutes. DNA ladders can
be visualized by agarose gel electrophoresis. The kit extracts
both genomic DNA and laddered DNA.
Provides a simple procedure
to prepare chromosomal DNA less than 90 minutes. DNA ladders can
be visualized by agarose gel electrophoresis. The new procedure
significantly improves sensitivity of detection with a unique
DNA staining dye, provided in the kit.
Provides a new procedure for
sensitive detection of Apoptotic DNA ladder. Apoptotic DNA
ladder can be easily visualized by agarose gel electrophoreses.
The kit specifically extracts laddered DNA, not genomic DNA.
Therefore, the kit can detect apoptotic DNA ladder from both
tissues and cells containing as little as 5% apoptotic cell
population.
Provides complete components
including positive and negative control cells for convenient
detection of DNA fragmentation in cultured cells by flow
cytometry.
Also provides complete
components including positive and negative control cells for
convenient detection of DNA fragmentation in cultured cells by
flow cytometry. The assay is suitable for GFP transfected cells.
Provides complete reagents
including positive and negative control slides for convenient
detection of DNA fragmentation in tissue sections and fixed cell
preps. Results can be analyzed by microscopy.
Provides complete components
including positive and negative control cells for convenient
detection of DNA fragmentation in cultured cells by flow
cytometry.
Provides a complete system
for separating nuclear fraction from the cytoplasm of mammalian
cells and tissues. Extracted fractions are compatible with
downstream assays.
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