Apurinic/apyrimidinic
(AP) sites are one of the major types of DNA lesions formed
during the course of base excision and repair of oxidized,
deaminated or alkylated bases. It has been
estimated that about 2x105 base lesions are
generated per cell per day. The level of AP sites in cells
can be a good indicator of DNA lesion and repair against
chemical damage and cell aging. The DNA Damage
Quantification Kit utilizes the ARP (Aldehyde Reactive
Probe) reagent that reacts specifically with an aldehyde
group which is the open ring form of the AP sites. After
treating DNA containing AP sites with ARP reagents, AP sites
are tagged with biotin residues, which can be quantified
using avidin-biotin assay followed by a colorimetric
detection. The kit provides the necessary reagents for
convenient determination of abasic sites in purified DNA
sample in 96-well plate format.
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1658 9045
+32
1650 9045
