|
Phase Service |
Timeline |
Description |
Price |
|
Phase 1: Subcloning |
1 Week |
Directly use customer’s clone and
expression system without any
modification |
No charge |
|
Subclone your desired cDNA into an
expression vector (Customer provides the
plasmid) |
500€ |
|
Confirm sequence of the final vector |
|
Subclone your desired cDNA into an
expression vector (plasmid provided by
RayBiotech) |
Inquire |
|
Confirm sequence of the final vector |
| |
|
Phase 2: Small-scale Protein Expression |
1 week |
Screen desired clones |
200€ |
|
Grow the clone in 100 mL of medium |
|
Express protein in 100 mL of medium |
|
Pellet cells, lyse cell pellet,
centrifuge and collect supernate |
|
Primary identification of protein
expression by immunological assay |
| |
|
Phase 3: Large-scale Protein Expression |
1 week |
Grow the selected clone in 2 L of medium |
400€ |
|
Express protein in 2 L of medium |
|
Pellet cells, lyse cell pellet,
centrifuge and collect supernate |
| |
|
Phase 4: Protein purification on a large
scale |
1 week |
Purify protein from supernates on
affinity chromatography column |
1,200€ |
|
Identify the fractions containing
desired protein using immunological
assay, such as Western Blot |
|
Concentrate the pooled fractions |
|
Determine the protein concentration |
|
Yield: Up to 100–500 µg of purified
protein |
|
Lyophilize protein to customer’s
specifications |
+32
1658 9045
+32
1650 9045
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